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KMID : 0368419890320020079
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Journal of Plant Biology
1989 Volume.32 No. 2 p.79 ~ p.87
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Isolation of Proteinase Inhibitor Ⅱ Genes from Potato
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Lee Jong-Seob
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Abstract
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Southern hybridization of genomic DNAs with radioactively labeled cDNA of tomato proteinase inhibitor Ⅱ revealed that proteinase inhibitor Ⅱ proteins in potato plants are encoded by a family of about 10 related sequences. Screening of potato EcoRI genomic library with the cDNA resulted in isolation of 13 recombinant phage clones which carry 3 different genomic regions. Of these clonse, clones 8, 18, and 39 were subjected to restriction mapping and subcloning. Further characterization of the subclones of clones 8, 18 and 39 indicated that two inhibitor Ⅱ genes are present on a 8.0 kb EcoRI fragment of clone 8, one on 3.3 and 0.8 kb EcoRI fragments of clone 18 and two genes on a 13.5 kb EcoRI fragment of clone 39.
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